食品科学2012，Vol.33Issue(5)：193-198,6.
单亲灭活德氏乳杆菌和乳酸乳球菌原生质体融合条件优化
Optimization of Conditions for Protoplast Fusion between Single Inactivated Lactobacillus delbrueckii and Lactococcus lactis
摘要
Abstract
Single inactivated protoplast fusion technique was used for the fusion of Lactobacillus delbrueckii FQ with Lactococcus lactis FL. The protoplast formation, regeneration and fusion conditions were examined in this study. Results showed that the optimal enzymolysis conditions for L. delbrueckii FQ were ultrasound treatment for 90 rain at 37℃ in the presence of 10 μ g/mL mutanolysin and 1 mg/mL lysozyme. Under these conditions, the protoplast regeneration rate reached 6.36%. The protoplast formation rate of L lactis FL was up to 99.97% after 90 min of treatment with 10 mg/mL lysozyme in the presence of 1 mg/mL glycine. L lactis FL protoplasts showed an inactivated rate of 96.89% after 120 rnin of exposure to 65℃. The fusion rate between Lactobacillus delbrueckii FQ and Lactococcus lactis FL reached 2.72 × 10^-6 after 5 rain of infusion at pH 6.5 in the presence of 400 g/L PEG 6000 （included 0.02 mol/L MgCh and 0.01 mol/L CaCl2）.关键词
单亲灭活/德氏乳杆菌/乳酸乳球菌/原生质体融合Key words
single inactivation: Lactobacillus delbrueckii/Lactococcus lactis/protoplast fusion分类
生物科学引用本文复制引用
李丽,房杰,黄洁洁,付瑞燕..单亲灭活德氏乳杆菌和乳酸乳球菌原生质体融合条件优化[J].食品科学,2012,33(5):193-198,6.基金项目
国家“973”计划项目 （）
安徽农业大学稳定和引进人才科研资助项目 （）
