食品科学2012，Vol.33Issue(5)：208-212,5.
响应曲面法优化酶解条件制备乳源ACE抑制肽
Response Surface Methodology for Optimization of Hydrolysis Conditions for the Production of Milk-derived Angiotensin-I Converting Enzyme （ACE） Inhibitory Peptides
摘要
Abstract
In our present study, response surface methodology was used to optimize process conditions for the hydrolysis of sodium caseinate by trypsin to prepare highly active angiotensin-I converting enzyme （ACE） inhibitory peptides. ACE inhibi- tory rate was measured by RP-HPLC. One-factor-at-a-time method followed by response surface analysis was used to analyze the effects of pH, temperature, hydrolysis time, substrate concentration, enzyme/snhstrate ratio on ACE inhibitory activity of sodium caseinate hydrolysate. A mathematical model describing the relationship of ACE inhibitory activity of sodium caseinate hydrolysate with temperature （A）, hydrolysis time （B）, substrate concentration （C） and enzyme/substrate ratio （/9） was obtained as follows： Y= -11.21347＋4.32902A-1.45953B＋3.42928C-0.20303D＋0.050303AB＋0.047422AD＋0.14955BC＋0.12486BD-0.054526A^2-0.079754B^2-0.53587C^2-0.28096D^2. The optimum hydrolysis conditions were determined as pH 7.0, 52.31℃, 19.44 h, substrate concentration of 5.91 g/100 mL and enzyme/substrate ratio of 8.37 %0, resulting in an ACE inhibitory rate of 97.11%.关键词
酪蛋白酸钠/胰蛋白酶/ACE抑制肽/响应曲面法/RP-HPLCKey words
sodium caseinate/trypsin/ACE inhibiting peptides/response surface methodology/RP-HPLC分类
轻工纺织引用本文复制引用
徐鑫,蔡丽丽,刘国艳,何佳易,魏晓蕊,王之颖,张军..响应曲面法优化酶解条件制备乳源ACE抑制肽[J].食品科学,2012,33(5):208-212,5.基金项目
江苏省高校自然科学基础研究项目（08KJD550004）：扬州市.扬州大学合作基金项目 （）
江苏省科技型中小企业技术创新资金项目（BN2008207）~江苏省普通高校研究生科研创新计划项目 （）
