食品科学2012，Vol.33Issue(5)：234-237,4.
海藻酸钠固定灵芝细胞对胞外三萜类产量的影响
Immobilization of Ganoderma lucidum in Sodium Alginate Beads and Effect on ExtraceUular Triterpene Production
摘要
Abstract
In this study, Ganoderma lucidum were immobilized by sodium alginate and calcium chloride. Immobilization conditions of Ganoderma lucidum using entrapment method were studied. The effects of immobilization conditions on tdterpenes yield were also evaluated. The results showed the optimal conditions of immobilization of Ganoderma lucidum by sodium alginate were as follows： 4.5% of sodium alginate, 2.2% of calcium chloride, nature pH, 40 immobilized beads, 28 ℃, 110 r/min, cultured 12 days, the highest triterpenes yield was （49.53 ±1.37） × 10^-2 mg/mL. Immobilized cells had better acid and alkali resistance, higher triterpenes yield after long time culture than non-immobilized cell. It can be recycled five times.关键词
灵芝/三萜类/固定化/海藻酸钠/氯化钙Key words
Ganoderma lucidum/triterpenoids/immobilization/sodium alginate/calcium chloride分类
轻工纺织引用本文复制引用
董玉玮,汤步伟,苗敬芝,曹泽虹..海藻酸钠固定灵芝细胞对胞外三萜类产量的影响[J].食品科学,2012,33(5):234-237,4.基金项目
江苏省高校自然科学研究项目 （）
徐州工程学院校级科研项目 （）
