食品科学2012，Vol.33Issue(6)：59-64,6.
罗非鱼尾胶原蛋白的提取与鉴定
Extraction and Identification of Collagen from Tilapia Tail
摘要
Abstract
The remove of lipid-soluble pigments and extraction of collagen from tilapia tails were studied.In addition,collagen purity and type were analyzed.The highest pigment removal rate,2.85%（calculated on wet basis）,was obtained after 36 h of treatment with a mixture of chloroform,methanol and water（1：2：0.8,V/V） at a material-to-liquid ratio of 1：1.5（g/mL）.The optimal conditions for collagen extraction,as determined by orthogonal array optimization,were guanidine hydrochloride concentration 3 mol/L,pepsin dose 3.0%（calculated based on crude protein weight）,material-to-liquid ratio 1：50（g/mL）,and twice repeated extraction for 72 h each time.Under these conditions,the extraction efficiency of collagen was 4.96%（calculated on wet basis）.In tilapia tails,collagen accounted for 40.39% of total crude protein.The extract obtained contained 94.63% of collagen and displayed the characteristics of collagen typeⅠ.关键词
胶原蛋白/色素/提取/鉴定Key words
collagen/pigment/extraction/identification分类
轻工纺织引用本文复制引用
段宙位,申铉日,陈秀明,王章晗..罗非鱼尾胶原蛋白的提取与鉴定[J].食品科学,2012,33(6):59-64,6.基金项目
教育部留学回国人员科研启动基金项目 （）
海南省自然科学基金项目 （）
