食品科学2012，Vol.33Issue(6)：166-170,5.
杜仲叶中松脂醇二葡萄糖苷的提取分离及含量测定
Extraction,Separation and Content Determination of Pinoresinol Diglucoside in Eucommia Leaves
摘要
Abstract
Pinoresinol diglucoside（PDG） from fresh eucommia leaves was prepared by ultrasonic-assisted solvent extraction at 60 ℃ for 30 min using 85% ethanol as the extractant at a solid-to-liquid ratio of 1：4（g/mL）,vacuum filtration,and thin layer chromatographic separation,resulting a yield of 2.00%.The PDG standard was found to have a maximum absorption at 228 nm.Therefore,the wavelength was used to detect PDG in extracts fresh eucommia leaves by HPLC.A good linear relationship was found between peak area and PDG concentration in the range of 0.00238-0.0950 mg/mL（y=84.8x＋0.838,r=0.9993）.The average spike recovery of PDG in a known sample was 102%.The PDG content in fresh eucommia leaves was roughly 0.0960% and the extract obtained by the method contained 4.54% PDG.The method was simple to operate,solvent saving and effective in extracting and separating PDG in eucommia leaves.关键词
杜仲叶/松脂醇二葡萄糖苷/提取/分离/含量测定/薄层色谱/高效液相色谱Key words
Eucommia ulmoides Oliv.leaf/pinoresinol diglucoside/extraction/separation/content determination/thin layer chromatography(TLC)/high performance liquid chromatography(HPLC)分类
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郑雪,刘泽纬,丁来欣,宋先亮..杜仲叶中松脂醇二葡萄糖苷的提取分离及含量测定[J].食品科学,2012,33(6):166-170,5.基金项目
北京林业大学国家大学生创新性实验计划项目 （）
农业科技成果转化资金项目 （）
