食品科学2012，Vol.33Issue(7)：215-218,4.
高含量己糖玉米芯纤维素酶解液发酵产酒精的研究
Ethanol Production by Fermentation of Corncob Hydrolysate with High Ratio of Hexose Prepared with Cellulase
摘要
Abstract
Corncobs were crushed into 1-2 cm^3, moistened by spraying 15g/100mL sulfuric acid, extracted for the removal of pentose, and then subjected to three-step countercurrent enzymatic hydrolysis and extraction. Hydrolysate with a hexose content of 10.26 g/100 mL was obtained. The fermentation of the hydrolysate by Saccharomyces cerevisiae HF2.0 for ethanol production was investigated. Placktt-Burman （PB） design was used to analyze the effects of MgSO4, KH2PO4, CaC12, soybean meal hydrolysate, wheat bran hydrolysate, and yeast mud added to the hydrolysate on ethanol production. MgSO4, KH2PO4 and soybean meal hydrolysate were identified as the main influential factors. As determined by steepest ascent tests and central composite design, the best fermentation results were obtained when the concentrations of MgSO4, KH2PO4 and soybean meal hydrolysate were 0.045, 0.135 g/100 mL and 7.34 g/100 mL, respectively, resulting in an ethanol yield of 88.64 g/L.关键词
己糖/玉米芯/酶解/乙醇发酵Key words
hexose/corn cob/enzymatic hydrolysis/ethanol fermentation分类
生物科学引用本文复制引用
赵辉,王海吉,王葳,凌宏志,平文祥..高含量己糖玉米芯纤维素酶解液发酵产酒精的研究[J].食品科学,2012,33(7):215-218,4.基金项目
哈尔滨市科技局科技创新人才专项 （）
黑龙江省教育厅科学技术研究项目 （）
