食品科学2012，Vol.33Issue(7)：237-241,5.
应用渗透膜高密度培养富硒螺旋藻
Application of Osmotic Membrane for High Cell Density Culture of Selenium-Enriched Spirulina platensis
摘要
Abstract
Selenium-enriched Spirulinaplatensis （Se-SP） was cultured in osmotic membrane tube （OT） and compared with air blowing bioreactor （AB） and shaking bottle （SB） cultures under the same conditions. Se supplementation was 100 μ g/mL in the form of sodium selenite. Growth rate and maximum biomass were monitored and deduced according to dry weight at designated time points. Total Se, organic Se and inorganic Se in Se-SP as well as soluble and insoluble Se species in culture medium were determined using a fluorimeter. Total protein （TP）, phycocyanin （PC）, chlorophyll a （Chla）, carotenoids （Caro）, total polysac- charides （SPS） and sulfydryl groups （-SH） in Se-SP were also detected colorimetrically. The contents of inorganic carbon （IC） and dissolved organic carbon （DOC） in culture medium were measured using a TOC-5000A analyzer. The results showed that the maximum biomass of Se-SP was up to 9.6 g/L during cultivation in OT tube, which was increased by 3- 5 fold compared with AB and SB cultures. The conversion rate of organic Se and the contents of TP, PC, Chla, SPS and SH group in Se-SP from OT culture were significantly higher than those from AB and SB cultures. Accordingly, OT culture produced lower IC residues and less accumulation of DOC, and higher soluble Se in the conditioned culture medium. In conclusion, OT can be used as a novel material for high cell density culture of Se-PS and it will have the advantages of high cell proliferation rate, high biotransformation of organic Se, high consumption of inorganic nutritional sources, low accumulation of organic carbon and less release of waster water.关键词
螺旋藻/富硒/高密度培养Key words
Spirulina platensis/selenium/high density culture分类
轻工纺织引用本文复制引用
任璐艳,靳兴媛,周永林,张逸波,凌钦婕,黄峙..应用渗透膜高密度培养富硒螺旋藻[J].食品科学,2012,33(7):237-241,5.基金项目
国家自然科学基金项目 （）
