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5种枣果实中凝集素的提取及生物活性分析

韩红艳 冀爱青 刘林敬 申奥

食品科学2012,Vol.33Issue(7):270-274,5.
食品科学2012,Vol.33Issue(7):270-274,5.

5种枣果实中凝集素的提取及生物活性分析

Extraction and Biological Activity Analysis of Lectin from Five Cultivars of Chinese Jujube

韩红艳 1冀爱青 1刘林敬 1申奥2

作者信息

  • 1. 晋中学院生物科学与技术学院,山西榆次030600
  • 2. 天津师范大学生命科学学院,天津300387
  • 折叠

摘要

Abstract

Lectin from 5 culfivars of Chinese jujube was isolated by ammonium precipitation and dialysis. Its ability to induce blood cell aggregation was then evaluated using different blood types (A, B and O). The results obtained showed that lectin from different cultivars of Chinese jujube had different aggregation-inducing activities for different blood types. For example, the blood cell aggregation valence to blood type A of lectin from Chinese jujube cultivars Huping, HuangHetan and Dabailing reached 2^7 compared with 2^5 and 2^6 for lectin from Chinese jujube cultivars Jun and Huping dates, respectively. Lectin from five different Chinese jujube cultivars were all stable at 70℃. However, when the temperature reached 80 ℃, all of them lose aggregation-inducing ability. Moreover, they had no monosaccharide and oligosaccharide specific binding sites. The metal ions Mg^2+, K^-, Cu^2+ and Fe^3+ were not essential for its blood cell aggregation activity.

关键词

枣果实/凝集素/血细胞/生物活性

Key words

jujube fruit/lectin/blood cells/biological activity

分类

农业科技

引用本文复制引用

韩红艳,冀爱青,刘林敬,申奥..5种枣果实中凝集素的提取及生物活性分析[J].食品科学,2012,33(7):270-274,5.

食品科学

OA北大核心CSCDCSTPCD

1002-6630

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