食品科学2012,Vol.33Issue(7):270-274,5.
5种枣果实中凝集素的提取及生物活性分析
Extraction and Biological Activity Analysis of Lectin from Five Cultivars of Chinese Jujube
韩红艳 1冀爱青 1刘林敬 1申奥2
作者信息
- 1. 晋中学院生物科学与技术学院,山西榆次030600
- 2. 天津师范大学生命科学学院,天津300387
- 折叠
摘要
Abstract
Lectin from 5 culfivars of Chinese jujube was isolated by ammonium precipitation and dialysis. Its ability to induce blood cell aggregation was then evaluated using different blood types (A, B and O). The results obtained showed that lectin from different cultivars of Chinese jujube had different aggregation-inducing activities for different blood types. For example, the blood cell aggregation valence to blood type A of lectin from Chinese jujube cultivars Huping, HuangHetan and Dabailing reached 2^7 compared with 2^5 and 2^6 for lectin from Chinese jujube cultivars Jun and Huping dates, respectively. Lectin from five different Chinese jujube cultivars were all stable at 70℃. However, when the temperature reached 80 ℃, all of them lose aggregation-inducing ability. Moreover, they had no monosaccharide and oligosaccharide specific binding sites. The metal ions Mg^2+, K^-, Cu^2+ and Fe^3+ were not essential for its blood cell aggregation activity.关键词
枣果实/凝集素/血细胞/生物活性Key words
jujube fruit/lectin/blood cells/biological activity分类
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韩红艳,冀爱青,刘林敬,申奥..5种枣果实中凝集素的提取及生物活性分析[J].食品科学,2012,33(7):270-274,5.