食品科学2012，Vol.33Issue(7)：275-279,5.
饲喂羊肉和鸭肉对大鼠结肠AQP2表达的影响
Effect of Diet Supplemented with Sheep Meat or Duck Meat on AQP2 Expression in Colon of Rats
摘要
Abstract
Objective： To evaluate AQP2 mRNA transcription and protein expression levels in rats fed die supplemented with sheep meat or duck meat. Methods： Totally 90 three-week-old male SD rats were randomly divided into three groups, and fed a diet supplemented with soybean protein （control）, sheep meat or duck meat, respectively. On the 14^th and 28^th day, serum antidiuretic vasopressin （AVP）, aquaporin-2 （AQP2） mRNA transcription, AQP2 protein expression, and Na^＋-K^＋-ATP enzyme activity were determined. Results： The rats fed sheep meat had higher levels of AVP, Na^＋-K^＋-ATP enzyme activity, AQP2 mRNA transcription and protein expression than those fed duck meat. Significant differences in other indices except protein expression level between sheep meat group and duck meat group were observed. In addition, both feeding groups on the 14~ and 28~ day also showed a significant difference in body weight. Conclusion： Sheep meat and duck meat have different effects on AQP2 mRNA transcription and protein expression. Sheep meetfortified diet reveals a positive effect, while the effect of duck meatfortified is negative. Therefore, sheep meat-fortified diet can limit the release of water, whereas duck meatfortified diet can increase the release of water.关键词
大鼠/饲喂羊肉/饲喂鸭肉/结肠/AQP2/荧光定量PCR/Western/BlotKey words
SD rats/feeding sheep meat/feeding duck meat/colon/AQP2/real time quantitative PCR/Western Blot分类
轻工纺织引用本文复制引用
闫振国,王思丹,王虎虎,徐幸莲,舒蕊华,董洋,周光宏..饲喂羊肉和鸭肉对大鼠结肠AQP2表达的影响[J].食品科学,2012,33(7):275-279,5.基金项目
公益性行业（农业）科技专项资金项目 （）
国际科技合作计划项目 （）
