食品科学2012，Vol.33Issue(7)：284-288,5.
藏灵菇源干酪乳杆菌KLl胞外多糖抑制人结肠癌细胞增殖的研究
Inhibitory Effect of Exopolysaccharides Produced by Lactobacillus casei Strain KL1 from Tibetan Kefir on Proliferation of Human Colon Cancer Cells
摘要
Abstract
In this study, exopolysaccharides （EPS） produced by Lactobacillus casei strain KL1, isolated from Tibetan Kefir, were purified and used to explore the effect on the proliferation of human colon cancer HCT-8 cells. The EPS was purified by Sepharose CL-6B gel column and its purity was evaluated by UV scanning spectroscopy and phenol-sulfuric acid assay. The cell proliferation and apoptotic effect of EPS on HCT-8 cells was examined by CCK-8 assay and Annexin V-FITC apoptosis detection kit. Two individual components （EPSa and EPSb） were obtained under the optimal purification conditions： gradient elution with 0.02-0.12 mol/L phosphate buffer at a flow rate of 3 mIdmin, and sample concentration of 15.0 mg/mL with a loading volume of 2.0 mL. The purities of EPSa and EPSb were 91.67% and 82.86%, respectively. EPSa could inhibit HCT-8 cell proliferation and induce cell apoptosis. The inhibitory rate of cell growth was in a dose-dependent manner. Therefore, EPSa reveals therapeutic effect on colon cancer.关键词
干酪乳杆菌/胞外多糖/HCT-8人结肠癌细胞/抑制增殖/细胞凋亡Key words
Lactobacillus casei/exopolysaccharides/HCT-8 human colon cancer cell/cell proliferation inhibition/cell apoptosis分类
轻工纺织引用本文复制引用
邢书涵,连正兴,孙勇,韩红兵,钟思琼,刘慧..藏灵菇源干酪乳杆菌KLl胞外多糖抑制人结肠癌细胞增殖的研究[J].食品科学,2012,33(7):284-288,5.基金项目
北京市自然科学基金项目（5092008 （）
5062006） （）
北京市重点建设学科资助项目 （）
