食品科学2012，Vol.33Issue(8)：109-114,6.
超高压结合酶法消减南美白对虾蛋白过敏原研究
Elimination of Allergens from Litopenaeus vannamei by Ultra High Pressure Treatment Combined with Enzymolysis
谢丹丹 1胡志和 1薛璐 1张博洋 1宿文晶 1方缘1
作者信息
- 1. 天津市食品生物技术重点实验室,天津商业大学生物技术与食品科学学院,天津300134
- 折叠
摘要
Abstract
Three methods,ultra high pressure（UHP） treatment,UHP treatment followed by papain hydrolysis and concurrent UHP treatment and papain hydrolysis,were developed for eliminating allergens in water soluble protein extracted from Litopenaeus vannamei.One-factor-at-a-time and orthogonal array design methods were used to optimize the operating conditions.The effectiveness of the methods was examined by indirect enzyme-linked immunosorbent assay（ELISA）.The optimal UHP treatment was performed under 150 MPa and for 35 min at 45 ℃ when used alone,and the OD492 nm of antigen-antibody reaction was 0.1997.When used before papain hydrolysis,the optimal UHP treatment was performed under 350 MPa for 20 min at 45 ℃,and the OD492 nm of antigen-antibody reaction was 0.0492.When used concurrently with papain hydrolysis,the optimal UHP treatment was performed under 300 MPa for 35 min at 45 ℃,and the OD492 nm of antigen-antibody reaction was 0.05.These results demonstrate that UHP treatment followed by papain hydrolysis and their concurrent action were more effective in eliminating allergens in Litopenaeus vannamei.Therefore UHP has eliminating effect on allergens in Litopenaeus vannamei.关键词
南美白对虾/高压法结合酶法/过敏原消减/酶联免疫Key words
Litopenaeus vannamei/UHP treatment combined with enzymolysis/allergen elimination/ELISA分类
轻工纺织引用本文复制引用
谢丹丹,胡志和,薛璐,张博洋,宿文晶,方缘..超高压结合酶法消减南美白对虾蛋白过敏原研究[J].食品科学,2012,33(8):109-114,6.
