食品科学2012，Vol.33Issue(8)：176-179,4.
离子色谱法测定奶粉中的葡萄糖、蔗糖和乳糖
Determination of Glucose,Sucrose and Lactose in Milk Powder by Ion Chromatography
摘要
Abstract
A high performance anion exchange chromatographic-pulse amperometric detection method was established to determine glucose,sucrose and lactose in milk powder.The separation column used was METROSEP CARB 1（150 mm × 4.0 mm） anion exchange column,and the mobile phase was 37 mmol/L NaOH.Citric acid was used to precipitate proteins from milk.The linear ranges of glucose,sucrose and lactose were 1-40,1-50 mg/L and 1-50 mg/L with correlation coefficients of 0.9966,0.9968 and 0.9985,respectively,and the detection limits were 0.014,0.091 mg/L and 0.083 mg/L.The precision relative standard deviations of precision for 5 replicate determinations were in the range of 1.10%-4.96%.The recovery rates for glucose,sucrose and lactose in 4 different commercial samples were 90.13%-104.83%.The method was suitable for rapid determination of glucose,sucrose and lactose in milk powder,thanks to its short analysis time and simple pretreatment.关键词
高效阴离子交换色谱/脉冲安培检测/奶粉/葡萄糖/蔗糖/乳糖/柠檬酸Key words
high performance anion exchange chromatography/pulsed amperometric detection/milk powder/sugar/citric acid分类
化学化工引用本文复制引用
熊建飞,周光明,许丽,张磊..离子色谱法测定奶粉中的葡萄糖、蔗糖和乳糖[J].食品科学,2012,33(8):176-179,4.基金项目
重庆市自然科学基金项目 （）
国家科技部重大专项 （）
