食品科学2012，Vol.33Issue(8)：207-210,4.
柱前衍生高效液相色谱法测定水产品中羟脯氨酸含量
Determination of Hydroxyproline in Fishery Products by Pre-Column Derivatization-High Performance Liquid Chromatography
摘要
Abstract
A high performance liquid chromatographic method was established for the determination of hydroxyproline in fishery products with pre-column derivatization.Samples were hydrolyzed with hydrochloric acid before pre-column derivatization with dansyl chloride.The analyze was separated using a C18 column and detected using a UV detector.Good separation was achieved for hydroxyproline and other amino acids.The linear range was 0.5-20 μg/mL（r 0.9999）.The detection limit was 0.5 μg/mL.The average spike recovery rates were 80.2%-103% with a RSD of 3.2%-5.6%.The method was sensitive,accurate and suitable for the determination of hydroxyproline in fishery products.关键词
羟脯氨酸/丹酰氯/柱前衍生/高效液相色谱法/水产品Key words
hydroxyproline/dansyl chloride/pre-column derivatization/HPLC/fishery products分类
化学化工引用本文复制引用
黄会,宫向红,刘慧慧,徐英江,李佳蔚,张秀珍..柱前衍生高效液相色谱法测定水产品中羟脯氨酸含量[J].食品科学,2012,33(8):207-210,4.基金项目
山东省水生动物营养与饲料泰山学者岗位资助项目 （）
国家海洋公益性行业科研专项（200805031 （）
200905019 （）
201105013） （）
