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中高温大曲和曲房细菌群落的相关性

王涛 游玲 赵东 冯瑞章 王松 冯学愚 张云

食品与发酵工业2012,Vol.38Issue(1):8-14,7.
食品与发酵工业2012,Vol.38Issue(1):8-14,7.

中高温大曲和曲房细菌群落的相关性

Correlation Between Bacterial Community of High-temperature DaQu and Air of Qu Workshop

王涛 1游玲 2赵东 3冯瑞章 1王松 1冯学愚 2张云4

作者信息

  • 1. 宜宾学院生命科学与食品工程学院,四川宜宾644000/发酵资源与应用四川省高校重点实验室,四川宜宾644000
  • 2. 发酵资源与应用四川省高校重点实验室,四川宜宾644000
  • 3. 五粮液股份公司,四川宜宾644000
  • 4. 宜宾学院生命科学与食品工程学院,四川宜宾644000
  • 折叠

摘要

Abstract

In order to better understand the correlation between bacterial community of high-temperature DaQu and air of Qu workshop of multi-grain strong-flavor liquor factories, and to collect potential microbial resources, we employed modified nutrient agar medium and Gogan-I medium for isolation and cultivation. Then 16S rRNA gene sequences of 217 non-redundant bacterial isolates were be analyzed, and abilities of lignocellulose-degrading, saccharif- ying, 7 % ethanol and pH 4. 5 tolerance of these isolates were be screened, too. Phylogenetic analysis based on 16S rRNA gene sequences showed that Bacillus is the most dominant genera of two bacterial community, and Staphylococcus and Streptomyces is following dominant genera of high-temperature DaQu and air of Qu workshop, respectively. These strains with abilities of lignocellulose-degrading, saccharifying, ethanol tolerance and structure of two bacterial communities were comparatively analyzed. The results indicated that Qu workshop bacterial community and climatic factors remarkably influenced the structure and function of high-temperature DaQu bacterial community.

关键词

中高温大曲/曲房/细菌群落/16S/rRNA基因/系统发育分析

Key words

high-temperature DaQu/Qu workshop/bacterial community/phytogenetie analysis/16S rRNA gene

分类

轻工纺织

引用本文复制引用

王涛,游玲,赵东,冯瑞章,王松,冯学愚,张云..中高温大曲和曲房细菌群落的相关性[J].食品与发酵工业,2012,38(1):8-14,7.

基金项目

四川省科技厅支撑计划项目 ()

四川省科技厅应用基础项目 ()

四川省教育厅再大培育项目 ()

四川省属高校白酒生产技术创新团队建设计划资助 ()

宜宾市科技专项研究项目 ()

食品与发酵工业

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