食品与发酵工业2012，Vol.38Issue(1)：20-24,5.
磷酸盐对大肠杆菌发酵异亮氨酸的影响
Effect of Phosphate on the Process of Escherichia coli L-isoleucine Fermentation
摘要
Abstract
The effects of phosphate on the fermentation of L-isoleucine was studied with L-isoleucine producing strain E.coli TRFC.The effects of initial phosphate concentration in the fermentation process on biomass,the specific growth rate,yield of L-isoleucine,concentration of byproduct acetic acid and ammonium were studied by carrying out fed-batch fermentation of E.coli TRFC in 30-Liter fermentor.The results showed that maintaining the initial phosphate at the concentration of 2g/L and adding 2g/L phosphate into the broth in the late period of fermentation could effectively slow down bacterial recession.The biomass and production of L-isoleucine increased by 24.5% and 12.7%,while the amount of by-product acetic acid decreased significantly.关键词
磷酸盐/大肠杆菌/比生长速率/异亮氨酸Key words
phosphate/Escherichia coli/the specific growth rate/L-isoleucine分类
化学化工引用本文复制引用
孙家凯,吴晓娇,王晶,徐庆阳,谢希贤,陈宁..磷酸盐对大肠杆菌发酵异亮氨酸的影响[J].食品与发酵工业,2012,38(1):20-24,5.基金项目
国家发改委高技术产业化专项项目：年产1 500吨支链氨基酸产业化示范工程 （）
