食品与发酵工业2012，Vol.38Issue(1)：36-40,5.
BAT2缺失酿酒酵母基因工程安全菌株的构建及其杂交育种
Construction and Crossing Trial of BAT2 Deletion Mutants Lacking Antibiotic Resistant Gene in Saccharomyces cerevisiae
摘要
Abstract
In order to obtain an engineering strain lacking antibiotic resistant gene with low-yield of higher alcohols,the plasmid pGAPZA with the gene encoding the Cre recombinase was transformed into the BAT2 deletion haploid mutants A8-B and C22-B.Then the G418 resistance gene（KanMX） was wiped off by Cre/loxp system from the mutants A8-B and C22-B,and Saccharomyces cerevisiae haploid mutants ΔBAT2a and ΔBAT2α were obtained.These two haploids were mated into a diploid ΔBAT2,which was verified to be genetically stable.Moreover,at the end of the liquor fermentation,the main fermentation profiles of the wild-type strain AY15,the parental strains A8-B and C22-B and the engineering strain ΔBAT2 were determined.The experiment results showed that the engineering strain ΔBAT2 was compared with the parental strains,weight loss of CO2 was higher,the residual total sugar was lower,ethanol was higher,yet the engineering strain was compared with the wild-type strain,the amount of isobutyl alcohol,isoamyl alcohol and the total higher alcohols were respectively reduced by 50.00%,33.40% and 30.57%.The engineering strain lacking antibiotic resistant genes with low-yield of higher alcohols was used as an excellent strain in the liquor industry.关键词
酿酒酵母/高级醇/Cre/loxp/G418/白酒发酵Key words
Saccharomyces cerevisiae/higher alcohols/Cre/loxp/G418/liquor fermentation分类
轻工纺织引用本文复制引用
张艳英,肖冬光,张翠英,王文阳,汪东升,李月强..BAT2缺失酿酒酵母基因工程安全菌株的构建及其杂交育种[J].食品与发酵工业,2012,38(1):36-40,5.基金项目
天津市科委应用基础及前沿技术研究计划重点项目资助 （）
