食品与发酵工业2012，Vol.38Issue(1)：96-100,5.
红曲霉ZL307固态发酵豆渣产γ-氨基丁酸的工艺优化
Optimization of Solid-state Fermentation Conditions for Production of γ-aminobutyric Acid by Monascus ZL307
摘要
Abstract
In order to reduce the production cost of γ-aminobutyric acid and make the best of bean dregs,the fermentation conditions for Monascus ZL307 to produce γ-aminobutyric acid with bean dregs as raw material in solid-state fermentation were optimized.Based on the study of single factor tests,we used Plaekett-Burman design to evaluate the influence of six related factors.Rice flour,MgSO4,KH2PO4 were affirmed the important factors in the medium.After identifying three critical factors,the optimal region of γ-aminobutyric acid production was found by steepest ascent approach.Then response surface methodology was also used to develop a mathematical model to identify the optimum concentrations of the key nutrients for higher γ-aminobutyric acid production,and to confirm its experimental validity.The results showed that the optimal fermentation conditions were as follows： bean dregs 12.28g,rice flour 7.72 g,（NH4）2SO4 0.20%,MgSO4 0.23%,KH2PO4 0.37%,CaCl2 0.25%,sodium glutamate 0.45%,initial water content 60%,initial pH 5.5,temperature 32 ℃.Under the optimum conditions,the yield of γ-aminobutyric acid reached 0.417 mg/g,which was 13.4% higher than before.关键词
红曲霉/γ-氨基丁酸/固态发酵/优化Key words
Monascus/γ-aminobutyric acid/solid-state fermentation/optimization分类
轻工纺织引用本文复制引用
张庆庆,吕闻闻,汤文晶,许鹏..红曲霉ZL307固态发酵豆渣产γ-氨基丁酸的工艺优化[J].食品与发酵工业,2012,38(1):96-100,5.基金项目
芜湖市重点科技项目 （）
