食品与发酵工业2012，Vol.38Issue(2)：207-211,5.
油菜蜂花粉水溶性蛋白的分离及其抗氧化活性
Study on Isolation and Antioxidant Activity Analysis of Water-soluble Protein from Rape Bee Pollen
摘要
Abstract
Rape bee pollen water-soluble proteins （RBPWPs） were isolated by the process of the ammonium sul- fate salting-out. The results showed that isolation with 20% - 40 % concentration of ammonium sulfate is the best, the yield and recovery were 711.54 Ixg/g and 29.91% , respectively. Also, antioxidative activities of RBPWPs with different concentration of ammonium sulfate precipitation （0% -20 % , 20% -40 % , 40% -60 % , 60% - 80 % and 80% - 100 % ） were determined in vitro. DPPH and · OH radical scavenging assay showed that RBPWPs with 20% - 40 % concentration of ammonium sulfate had the highest activity. Additionally, all RBPWPs scavenged ·OH radical activities were higher than DPPH. Combination with SDS-PAGE result, it indicated that RBPWPs with -39. 0 ku and ·58.0 ku molecular weight may play an important role in antioxidation reactions.关键词
油莱蜂花粉/水溶性蛋白/分离/抗氧化活性Key words
rape bee pollen/water-soluble protein/isolation/antioxidative activity分类
轻工纺织引用本文复制引用
邓建军,杨海霞,曹炜,程妮,王毕妮,高慧..油菜蜂花粉水溶性蛋白的分离及其抗氧化活性[J].食品与发酵工业,2012,38(2):207-211,5.基金项目
西安市农业应用技术研究项目（NC10018（18）） （）
陕西省科学技术研究发展计划项目 （）
西北大学校内基金 （）
