独活活性单体对中型指环虫的杀灭作用及其成分鉴定OA北大核心CSCDCSTPCD
EFFECT OF THE ACTIVE MONOMER FROM RADIXANGELICAE PUBESCENTIS ON KILLING DACTYLOGYRUS INTERMEDIUS AND ITS ACTIVE COMPONENT IDENTIFICATION
利用硅胶柱层析和生物活性跟踪对独活乙醇提取物进行杀中型指环虫活性成分的分离纯化,并测定活性成分在不同浓度条件下的离体生物活性,通过薄层色谱(TLC)、紫外光谱(UV)、高效液相色谱(HPLC)、气质联用(GC-MS)对活性成分进行鉴定.结果表明:从独活分离得到的F和K1-2-3两种流分均对中型指环虫具有不同程度的杀灭作用,其中F流分48h内的完全杀灭中型指环虫的浓度为1.6 mg/L,而K1-2-3的使用浓度分别为5 mg/L时,其48h内对中型…查看全部>>
Activated components of parasiticidal Dactylogyrus intermedius extracted from Radix angelicae pubescentis were isolated and purificated by column chromatography and biologica 1 tracking in this study. The activity in vitro of activated components at different concentrations were determined. Then their chemical structures were identified by TLC, UV, HPLC and GC-MS. Observations implied that the killing effect of the components on D. Intermedius at different c…查看全部>>
汪开毓;姚璐;谢嘉宾;黄锦炉;苗常鸿
四川农业大学鱼病研究中心,雅安625014四川农业大学动物疫病与人类健康四川省重点试验室,雅安625014四川农业大学鱼病研究中心,雅安625014四川农业大学鱼病研究中心,雅安625014四川农业大学鱼病研究中心,雅安625014
农业科技
中型指环虫独活活性成分杀虫活性成分鉴定
Dactylogyrus intermedius Radix angelicae pubescentis Active components Parasiticidal activity Com-ponent identification
《水生生物学报》 2012 (1)
93-101,9
农业部水生动物遗传育种和养殖生物学重点开放实验室开放研究基金资助课题(Bm2007-11)教育部《长江学者和创新团队发展计划》创新团队项目(IRT0848)资助
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