中国食品学报2012，Vol.12Issue(3)：34-38,5.
中华真地鳖抗氧化肽的分离及其抗氧化活性的研究
Study on Isolation and Antioxidant Activity of Antioxidant Peptide from Eupolyphaga Sinensis Walker
摘要
Abstract
The separation of antioxidant peptides from the enzymatic hydrolysate of Eupolyphaga sinensis Walker and the antioxidant activities of the peptides were investigated. Three fractions, including FI, FII, and Fill, were obtained by DEAE-Sephadex A 50 chromatogiaphy, of which, fraction FII exhibited the highest O2-·, OH·, and DPPH· scavenging capabilities, reaching 86.83% (10 mg/mL), 92.28% (0.7 mg/mL), and 68.06% (1.8 mg/mL), and the corresponding IC50s were 5.98 mg/mL, 0.27 mg/mL, and 1.09 mg/mL, respectively. Fraction FII was further purified by HPLC and the optimum resolution was yielded in acetonitrile concentration range of 0-30% and sample load of 1 mg.关键词
中华真地鳖/抗氧化肽/抗氧化Key words
Eupolyphaga sinensis Walker/ antioxidant peptide/ antioxidantion引用本文复制引用
王懋存,金玉兰,朴美子..中华真地鳖抗氧化肽的分离及其抗氧化活性的研究[J].中国食品学报,2012,12(3):34-38,5.基金项目
山东省自然科学基金项目(ZR2009DM017) （ZR2009DM017）
