饲料及动物性食品中山羊源性和绵羊源性成分双重荧光PCR检测方法的建立OA
Development of A Dual Fluorescent Real-Time PCR for the Detection of Mitochondrial DNA of Goat and Sheep Mixed in Feed and Food Products
目的建立一种能同时检测饲料及动物性食品中的山羊和绵羊源性成分的双重荧光PCR方法，应用于动物源性饲料及动物性食品的快速检验。方法分别根据山羊、绵羊线粒体种间保守序列，设计合成针对山羊和绵羊的特异性引物及TaqMan探针，通过荧光PCR反应体系和反应条件的优化筛选，建立可同时检测饲料及动物性食品中的山羊和绵羊源性成分的双重荧光PCR方法。结果本研究所建立的双重荧光PCR检测方法可同时快速检测饲料及动物性食品中的山羊和绵羊源性成分，应用本方法检测山羊…查看全部>>
Objective To develop a dual fluorescent real-time PCR assay for the simultaneous detection of goats and sheep-origined feed and goats and sheep-origined food products. Methods A dual fluorescent real-time PCR was developed using specific primers and TaqMan probes designed according to mitoehondrial DNA gene sequences, and screening by fluorescence PCR reaction system and the optimization of reaction conditions. Results The dual real-time PCR developed can de…查看全部>>
陈永锋
厦门市农产品质量安全检验测试中心,361012 厦门市动物疫病预防控制中心,361012
农业科技
线粒体双重荧光PCR山羊和绵羊源性成分
Mitochondria dual fluorescent real-time PCR goat and sheep-origined materials
《福建畜牧兽医》 2012 (3)
1-4,4
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