现代食品科技2012，Vol.28Issue(4)：405-408,440,5.
丙烯酰胺多克隆抗体的制备
Preparation ofAcrylamide Polyclonal Antibodies
摘要
Abstract
Rabbits were immunized with three kinds of acrylamide artificial antigens which were synthetized via active ester method, then the antibodies with the titer of 1:32000, 1:40000 and 1:8000 were obtained. High specific antibody was produced from acrylamide and 3-mercaptobenzoic acid (3-MBA). The inhibition rate of antibody against acrylamide derivative at the concentration of t μg/mL was 80.7%, and there was no cross-reactivity with acrylamide or 3-MBA. The research provided references for the rapid detection for acrylamide in processed food by ELISA method关键词
丙烯酰胺/人工抗原/多克隆抗体/衍生化Key words
artificial antigen/ polyclonal antibodies/ derivative引用本文复制引用
王宵雪,刘冰,吕燕彦,徐小婧,方国臻,王硕..丙烯酰胺多克隆抗体的制备[J].现代食品科技,2012,28(4):405-408,440,5.基金项目
"十二五"国家科技支撑计划项目(2012BAK17B03) （2012BAK17B03）
