华中师范大学学报（自然科学版）2012，Vol.46Issue(2)：195-197,203,4.
PI3K-Akt和JNK信号通路抑制剂对杆状病毒诱导昆虫细胞凋亡影响的初步研究
Effect of inhibitors of PI3K-Akt and JNK signaling pathway on apoptosis induced by baculovirus in spodoptera litura cells
摘要
Abstract
In order to explore the relationship between baculovirus-induced insect cell ap-optosis pathway and PI3K-Akt or JNK signaling pathways, we used the PI3K inhibitor Wortmannin and JNK inhibitor SP600125 to treat SL-1 cells infected with SfaMNPV and determined the effects of these inhibitors on apoptosis induced by SfaMNPV in SL-1 cells . After cells infected with AfMNPV in the presence of 2. 5,25,50 μmol SP600125 or 0. 3,3,30 μmol Wortmannin respectively, we observed the cells morphology and ap-optotic characterization by light microscope, DAPI staining and detected the level of apoptosis in Sl-1 cell with flowcytometry. The results showed that the inhibitors significantly inhibited apoptosis induced with AfMNPV in Sl-1 cells, suggesting that PI3K-Akt and JNK signaling pathway can involve in the apoptosis and the two signaling pathways play an important role during apoptosis induced with AfMNPV in Sl-1 cells.关键词
PI3K-Akt信号通路/JNK信号通路/细胞凋亡/抑制剂/杆状病毒Key words
PI3K-Akt signaling pathway/ JNK signaling pathway/ apoptosis/ inhibitor/ baculovirus分类
生物科学引用本文复制引用
张茜,彭建新,洪华珠..PI3K-Akt和JNK信号通路抑制剂对杆状病毒诱导昆虫细胞凋亡影响的初步研究[J].华中师范大学学报（自然科学版）,2012,46(2):195-197,203,4.基金项目
国家自然科学基金项目(30470073). （30470073）
