乳业科学与技术2012，Vol.35Issue(1)：40-42,3.
高效离子色谱法测定乳及乳制品中低聚果糖含量
Determination of Fructooligosaccharides in Milk and Milk Products by High Performance Ion Exchange Chromatography
刘玉峰 1李东 1唐华澄 1张绩觅1
作者信息
- 1. 北京市营养源研究所分析检测中心,北京100069
- 折叠
摘要
Abstract
An analytical method was established to determine fructooligosaccharides （FOS） in milk and milk products by high performance ion exchange chromatography method with pulsed amperometric detection. The column was a CarboPac PAl （4 mm x 250 mm） anion exchange column. Fructooligosaccharides were separated under gradient elution mode. The mobile phase A was 150 mmol/L NaOH and the mobile phased B was composed of 150 mmol/L NaOH and 100 mmol/L sodium acetate. The relative standard derivations for 6 replicate determinations of four fructooligosaccharides in liquid milk were less than 9%. The average spike recovery rates for three fructooligosaccharides in liquid milk varied between 94.8% and 103.6%. The method was simple, fast and suitable for determining FOS in milk and milk products.关键词
高效离子色谱法/脉冲安培检测/低聚果糖Key words
high performance ion exchange chromatography (HPIEC)/pulsed amperometric detection (PAD)/fructooligosaccharides (FOS)分类
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刘玉峰,李东,唐华澄,张绩觅..高效离子色谱法测定乳及乳制品中低聚果糖含量[J].乳业科学与技术,2012,35(1):40-42,3.
