食品工业科技2012，Vol.33Issue(11)：109-112,4.
纳豆菌脂肽对金黄色葡萄球菌抑菌机理的研究
Study on antibacterial mechanism of lipopeptide from Bacillus natto against Staphyloccocus aureus
摘要
Abstract
[Objective] The antibacterial mechanism of lipopeptide from Bacillus natto against Staphyloccocus aureus was studied. [Method] The antibacterial mechanism of lipopeptide from Bacillus natto against Staphyloccocus aureus was studied according to the effect of lipopeptide on the growth curves,membrane permeability,protein synthesis,the structure under scanning electron microscope,and respiratory metabolism. [Result] The lipopeptide could increase membrane permeability of Staphyloccocus aureus,which led the macromolecules with ultraviolet absorption in cell to leak out of the cell. Observation under scanning electron microscope revealed the holes were formed in the cell wall of Staphyloccocus aureus,and even the cell was ruptured by lipopeptide. SDS-PAGE analysis suggested that the lipopeptide had not apparent effects on proteins expression,but had some effects on synthesized quantities of some proteins. The respiratory metabolism of Staphyloccocus aureus was inhibited by lipopeptide. [Conclusion] The lipopeptide acted on the cell membrane of Staphyloccocus aureus,which induced membrane permeability of Staphyloccocus aureus increased,or holes were formed in the cell membrane,thus the materials in the cell to leak out of it,resulted in inhibiting the growth of cell ,and killing the cell.关键词
纳豆菌/脂肽/金黄色葡萄球菌/抑菌机理Key words
Bacillus natto lipopeptide Staphyloccocus aureus antibacterial mechanism分类
轻工纺织引用本文复制引用
刘唤明,孙力军,王雅玲,徐德峰,刘阳,陈宏,唐晓山..纳豆菌脂肽对金黄色葡萄球菌抑菌机理的研究[J].食品工业科技,2012,33(11):109-112,4.基金项目
国家自然科学基金 （）
