食品工业科技2012，Vol.33Issue(11)：179-182,186,5.
响应面法优化碱性蛋白酶提取菜籽饼中可溶性蛋白质的研究
Optimization of alkaline protease extraction technology of protein from rapeseed meal by response surface methodology
摘要
Abstract
Rapeseed meal was by-products of pressing rapeseed oil and a good resource of protein extraction. Using rapeseed meal as material,the technology for extracting rapeseed meal protein with alkaline protease was discussed. Based on systematic study of effect of liquid to solid ratio,enzyme dosage,value of initial pH, reaction temperature and reaction time on extraction rate of protein,main effect factors were optimized and optimum technology was received. When reaction time was constantly 40min,the result demonstrated extraction rate of protein was 55.38% under optimum conditions namely that enzyme dosage 6500U/g, reaction temperature 45℃,value of initial pil11 ,ratio of liquid to solid 17：1.关键词
菜籽饼蛋白/碱性蛋白酶/响应面法Key words
rapeseed meal protein alkaline protease response surface methodology分类
轻工纺织引用本文复制引用
汤务霞,冯程,韩艳..响应面法优化碱性蛋白酶提取菜籽饼中可溶性蛋白质的研究[J].食品工业科技,2012,33(11):179-182,186,5.基金项目
四川省教育厅青年基金项目 （）
