食品工业科技2012，Vol.33Issue(11)：253-255,263,4.
阴离子交换层析法分离纯化γ-PGA
Isolation and purification of y-PGA by anion exchange column chromatography
王浩 1赵祥颖 2田延军 2乔君 2刘建军3
作者信息
- 1. 山东轻工业学院食品与生物工程学院,山东济南250353
- 2. 山东食品发酵工程重点实验室,山东济南250013
- 3. 山东轻工业学院食品与生物工程学院,山东济南250353／山东食品发酵工程重点实验室,山东济南250013
- 折叠
摘要
Abstract
y-PGA was purified by anion exchange column chromatography. Through statical adsorption test and dynamic test of the anion resin,the optimum technological conditions were determined as follows.sample pH 8.0,sample quantity of resin 27.3mg/g ,eluant 0.7 mol/L NaCI,eluting velocity 1.0mL/min,and eluting temperature 20℃. At last,the purity of y-PGA was improved from 86.7% to 96.2%.关键词
γ-聚谷氨酸/静态吸附/分离纯化Key words
y-Poly glutamic acid/statical adsorption/separation and purification分类
轻工纺织引用本文复制引用
王浩,赵祥颖,田延军,乔君,刘建军..阴离子交换层析法分离纯化γ-PGA[J].食品工业科技,2012,33(11):253-255,263,4.
