食品工业科技2012，Vol.33Issue(11)：256-259,263,5.
酶-碱法提取碎米蛋白工艺研究
Optimization of enzyme-alkali assisted extraction of rice protein from broken rice
摘要
Abstract
Broken rice,byproduct of refined rice manufacture was used as raw material to extract its protein by enzymatic enrichment and alkali extraction methods. Firstly,co-amylase＇was applied to pretreat the broken rice for enriching protein elements. Under the condition that 95℃, pH7.5,solid-liquid ration 1：15,c c-amylase content 20U/g and hydrolysis time 150 min,liquefaction rate of broken rice was up to 61.4%,which was use for the next alkaline extraction after centrifugation. Based on the extraction rate of protein （BRP）,the single factor and orthogonal analysis method were designed to optimize alkaline extraction conditions. The results showed that the best parameter for protein extraction were as follows .. NaOH 0.06mol/L,solid-liquid ratio（g/mL） 1：5,extraction time 5h and extraction temperature 50℃. The extraction ration of BRP was up to 86.26% under the optimized process. Enzyme-alkali assisted extraction method integrated the enzymatic enriching and alkali extraction, which would provide some theoretical foundation for the production of BRP and the value-increment application of broken rice resources.关键词
碎米/碎米蛋白/酶-碱法提取/工艺优化Key words
broken rice broken rice protein enzyme-alkali assisted extraction technologic optimization分类
轻工纺织引用本文复制引用
赵世光,张焱,薛正莲,樊龙,项敏..酶-碱法提取碎米蛋白工艺研究[J].食品工业科技,2012,33(11):256-259,263,5.基金项目
安徽省高校省级自然科学项目 （）
安徽工程大学引进人才科研启动基金 （）
安徽省科技厅国际科技合作计划项目 （）
