食品工业科技2012,Vol.33Issue(11):333-337,5.
辣椒中辣椒素类物质的检测方法及稳定性研究
Study on determination of capsaicinoids in capsicum chilli and its storage stability
罗金凤 1张迪 1丁晓雯 1章道明 1梁菡峪1
作者信息
- 1. 西南大学食品科学学院,重庆市农产品加工重点实验室,重庆400716
- 折叠
摘要
Abstract
Purpose:The aim of this study was to find a faster way to detect capsaicin and dihydrocapsaicin through optimizing the detection chromatographic condition according to GB/T 21266-2007,and to evaluate their storage stability after extracted from fresh capsicum chilli as well. Method.HPLC. The chromatographic condition was using C18 column(4.6mm×250mm,5μm),with methanol:water(V:V=70:30) as mobile phase. The flow rate was 1.0mL/min and the detection wavelength was set to 280nm. The injection volume was 10μL and the column temperature was set to 30℃. Result=Compared to the Chromatographic conditions in GB/T 21266-2007,the detection time can reduce about 12 minutes. The contents of extracted capSaicin and dihydrocapsaicin were not fluctuating sharply within a month at room temperature. Conclusion:This chromatographic condition could effectively detect capsaicin and dihydrocapsaicin,and could obviously reduce capsaicin and dihydrocapsaicin retention time. The extracted capsaicin and dihydrocapsaicin could keep their stability within a short time during storage at room temperature.关键词
辣椒素/二氢辣椒素/稳定性/高效液相色谱/检测Key words
caDsaicin dihvdrocaiosaicin stability HPLC determi nation分类
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罗金凤,张迪,丁晓雯,章道明,梁菡峪..辣椒中辣椒素类物质的检测方法及稳定性研究[J].食品工业科技,2012,33(11):333-337,5.