食品工业科技2012，Vol.33Issue(12)：87-89,92,4.
壳寡糖制备过程中还原糖的全自动电化学测定
Automatic electrochemical determination of reducing sugar during the preparation of chitosan oligosaccharide
摘要
Abstract
Reducing sugar was measured using reducing sugar electrochemical analyzer during the preparation of chitosan oligosaccharide.The linear range of glucosamine was from 0.005% to 0.500%,and detection limit was 0.005%.The effect of different enzymatic conditions such as temperature,pH,substrate concentration on the reaction were researched by changes of reducing sugar content.The enzymatic hydrolysis process had been optimized.The relationship between the degree of polymerization of the product and the reducing sugar concentration was got.Reducing sugar electrochemical analyzer could determine reducing sugar of low concentrations during the preparation of chitosan oligosaccharide,it was fast,accurate,high sensitivity.关键词
壳寡糖/还原糖/电化学测定/酶解Key words
chitosan oligosaccharide/reducing sugar/electrochemical determination/enzymatic hydrolysis分类
轻工纺织引用本文复制引用
李雪梅,史建国,郑晖,马耀宏,李秋顺..壳寡糖制备过程中还原糖的全自动电化学测定[J].食品工业科技,2012,33(12):87-89,92,4.基金项目
山东省科技攻关项目 （）
山东省自然科学基金 （）
济南市青年科技明星计划 （）
山东省博士后专项创新基金 （）
