食品工业科技2012，Vol.33Issue(12)：93-96,4.
分子马达传感器对沙门氏菌快速检测方法的初步研究
Preliminary study of a rapid detecting technology for Salmonella based on molecular motor biosensor
摘要
Abstract
Using F0F1-ATPase of spin characteristics and H＋-sensitive fluorescent material F-DHPE as an indicator to detect the target sample.Specific invA probe were connected with F0F1-ATPase’s ε subunit by using avidin-biotin system,and then biosensors were constructed,the test sample and negative sample,respectively,combined with biosensors,to compare their catalytic ATP synthesis after 30min,Salmonella DNA in the samples can be tested.According to the result of our experiment,it took 1h and could detect accurately about 10ng/mL for standard strain.30 strains of food sample which were detected with the PCR detection results that were consistent.关键词
沙门氏菌/F0F1-ATP合酶/分子马达传感器/快速检测Key words
Salmonella/F0F1-ATPase/molecule motor biosensor/rapid detection分类
轻工纺织引用本文复制引用
张捷,顾德周,张惠媛,杨泽慧,王佩荣,张雷,齐玮,陈广全,乐加昌..分子马达传感器对沙门氏菌快速检测方法的初步研究[J].食品工业科技,2012,33(12):93-96,4.基金项目
国家质检总局科技计划项目 （）
