食品工业科技2012,Vol.33Issue(12):111-113,117,4.
鞣花酸和石榴皮多酚提取物抗氧化活性的比较
Comparative study of antioxidant activities of ellagic acid and pomegranate peel extract
摘要
Abstract
The comparative study was conducted on the antioxidant activities of ellagic acid extract(EA) and pomegranate peel extract(PE).The content of ellagic acid in PE and EA were detected by HPLC,and the content of total polyphenol in PE by folin-phenol.Oxidative stress experimental model of cells was established with HepG2 cell treated by H2O2.Then the MTT was employed to determinate the protective effects of various levels of PE and EA for HepG2 cell against oxidative stress injury.DPPH method and ·OH scavenging method were used to estimate the free radicles elimination ability in vitro of PE and EA.The result showed that the content of ellagic acid was much lower in PE than that in EA,but both PE and EA,in a dose-reliant way,increased the survival rate of HepG2 cells which were under oxidative stress injury-they demonstrated remarkable protective effect.At the same time,the protective effect of PE was markedly better than that of EA.The elimination rate of both PE and EA upon DPPH method and ·OH scavenging method was also in a dose-reliant fashion.关键词
石榴皮多酚/鞣花酸/HepG2细胞/H2O2/抗氧化Key words
PE/EA/HepG2 cells/H2O2/antioxidant activity分类
轻工纺织引用本文复制引用
饶夙,陈祥贵,刘振平,文春鹃,何宇新,杨文宇..鞣花酸和石榴皮多酚提取物抗氧化活性的比较[J].食品工业科技,2012,33(12):111-113,117,4.基金项目
国家星火计划项目 ()
四川省科技攻关项目 ()