食品工业科技2012，Vol.33Issue(12)：132-135,139,5.
氯化钠刺激对保加利亚乳杆菌生长及质膜流动性的影响
Effect of NaCl stimulation on growth and membrane fluidity of Lactobacillus bulgaricus in media
摘要
Abstract
Objective：Effect of NaCl stimulation on the growth,membrane fluidity and membrane fatty acid composition of Lactobacillus bulgaricus ATCC11842 were investigated.Methods：Fluorescent probe（1,6-diphenyl-1,3,5-hexatirene,DPH）were used to analyze the fluidity（membrane polarization）of the cellular cytoplasmic membrane after stimulation of different concentrations of NaCl levels.Meanwhile,Gas chromatography（GC）was used to analyze fatty acid composition of cytoplasmic membrane under the same conditions.Results：The maiximum tolerance of L.bulgaricus was up to 1.0mol/L NaCl.Major fatty acids of this bacterium were palmitic acid（16：0）and cis-vaccenic acid（18：1）under salt stimulation（0.4mol/L NaCl）.The salt concentration strongly influenced the fatty acid composition.the rate of saturation to unsaturation increased from 0.57 to 0.78 as the increasing of NaCl concentrations.Meanwhile,the growth of L.bulgaricus decreased.Conclution：When the concentration of NaCl was increased from 0.2mol/L to 0.8mol/L,the bacterial cytoplasmic membrane fluidity decreased with the fluorescence polarization increasing and the ratio of the fatty acid in cytoplasmic membrane increased.关键词
保加利亚乳杆菌/盐刺激/荧光探针/细胞膜流动性/脂肪酸Key words
Lactobacillus bulgaricus/salt stimulation/fluorescent probe/membrane fluidity/fatty acid分类
轻工纺织引用本文复制引用
刘丽波,陈婧,腾飞,刘宁,李春,刘景圣..氯化钠刺激对保加利亚乳杆菌生长及质膜流动性的影响[J].食品工业科技,2012,33(12):132-135,139,5.基金项目
国家教育部长江学者和创新团队发展计划 （）
东北农业大学博士启动基金 （）
