食品工业科技2012,Vol.33Issue(12):285-288,4.
复合酶法提取红雪茶粗多糖工艺优化研究
Optimization of polysaccharide extraction from Lethariella Cladonioides by compound enzymes method
摘要
Abstract
To obtain the optimized process parameter of extracting the polysaccharide from Lethariella Cladonioides,single factor experiment and orthogonal test were adopted.The effect of different solid-liquid ratio,pH,temperature of enzymatic hydrolysi(cellulase,Pectinase,papain),extracting time and compound enzymes mixture ratio on the extraction rate of polysaccharide from Lethariella Cladonioides were studied.The results showed that compound enzyme best ratio was 2.0% cellulase,2.0% pectinase,0.5% papain.The impact factors were as following:solid-liquid ratio〉temperature〉pH〉time.The optimized process parameter was solid-liquid ratio 1:40,pH4.5,temperature 40℃,time 80min.Under this condition the extraction rate of polysaccharide reached to 8.91%.This method was suitable for polysaccharide extraction from Lethariella Cladonioides.关键词
复合酶法/红雪茶/多糖/提取Key words
compound enzymes/Lethariella Cladonioides/polysaccharide/extraction分类
轻工纺织引用本文复制引用
杨蓉生,陈炼红,唐俊妮,陈娟,唐艳琼..复合酶法提取红雪茶粗多糖工艺优化研究[J].食品工业科技,2012,33(12):285-288,4.基金项目
中央高校基本科研业务费专项 ()