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复合酶法提取红雪茶粗多糖工艺优化研究

杨蓉生 陈炼红 唐俊妮 陈娟 唐艳琼

食品工业科技2012,Vol.33Issue(12):285-288,4.
食品工业科技2012,Vol.33Issue(12):285-288,4.

复合酶法提取红雪茶粗多糖工艺优化研究

Optimization of polysaccharide extraction from Lethariella Cladonioides by compound enzymes method

杨蓉生 1陈炼红 1唐俊妮 1陈娟 1唐艳琼1

作者信息

  • 1. 西南民族大学生命科学与技术学院,四川成都610041
  • 折叠

摘要

Abstract

To obtain the optimized process parameter of extracting the polysaccharide from Lethariella Cladonioides,single factor experiment and orthogonal test were adopted.The effect of different solid-liquid ratio,pH,temperature of enzymatic hydrolysi(cellulase,Pectinase,papain),extracting time and compound enzymes mixture ratio on the extraction rate of polysaccharide from Lethariella Cladonioides were studied.The results showed that compound enzyme best ratio was 2.0% cellulase,2.0% pectinase,0.5% papain.The impact factors were as following:solid-liquid ratio〉temperature〉pH〉time.The optimized process parameter was solid-liquid ratio 1:40,pH4.5,temperature 40℃,time 80min.Under this condition the extraction rate of polysaccharide reached to 8.91%.This method was suitable for polysaccharide extraction from Lethariella Cladonioides.

关键词

复合酶法/红雪茶/多糖/提取

Key words

compound enzymes/Lethariella Cladonioides/polysaccharide/extraction

分类

轻工纺织

引用本文复制引用

杨蓉生,陈炼红,唐俊妮,陈娟,唐艳琼..复合酶法提取红雪茶粗多糖工艺优化研究[J].食品工业科技,2012,33(12):285-288,4.

基金项目

中央高校基本科研业务费专项 ()

食品工业科技

OA北大核心CSCDCSTPCD

1002-0306

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