食品工业科技2012，Vol.33Issue(13)：67-70,4.
对虾传染性皮下及造血组织坏死病毒衣壳蛋白基因在毕赤酵母中的表达
Expression of the coat protein gene of IHHNV in shrimp in Pichia Pastoris
摘要
Abstract
The infectious hypodermal ＆ haematopoietic necrosis virus is one of the main diseases of the penaeid shrimp. The gene of coat protein of IHHNV was constructed into Pichia Pastoris secretory expression vectors pPIC9K,named pPIC9K-IV. The recombinant plasmid pPIC9K-IV was linearized with Bgl II,and then was transformed into Pichia Pastoris GS115 by electroporation. After PCR analysis and G418 screening,the pPIC9K- IV recombinant was expressed with 0.8% （VN） methanol. The expression product was confirmed by Western blot and ELISA analysis. The results showed that the coat protein was successfully expressed,its product was about 40ku,and could recognized specifically by rabbit polyclonal anti-serum.关键词
传染性皮下及造血组织坏死病毒/衣壳蛋白/毕赤酵母/真核表达Key words
infectious hypodermal & haematopoietic necrosis virus(IHHNV)/coat protein/Pichia Pastoris/eukaryoticexpression分类
轻工纺织引用本文复制引用
彭小莉,谢明星,刘棠,陈双雅,王群力,陈伟玲,王景明,张其清..对虾传染性皮下及造血组织坏死病毒衣壳蛋白基因在毕赤酵母中的表达[J].食品工业科技,2012,33(13):67-70,4.基金项目
国家质检总局科技基金资助项目（2006IK015）. （）
