食品工业科技2012，Vol.33Issue(13)：110-113,4.
药食两用中药中α-葡萄糖苷酶抑制剂的高通量筛选
High throughput screening of α-glucosidase inhabitors in edible traditional Chinese medicine
摘要
Abstract
Objective：To screen α-glucosidase inhibitors by high throughput way from edible traditional Chinese medicine （ETCM）. Methods ： α-Glucosidase in the petroleum ether, dichloromethane, ethyl acetate, methanol, water extracts from 72 species of ETCM were assayed based on the concentration of PNP in the reaction system by HPLC using PNPG as substrate. Results.It was found that most of extracts at the concentraction of 0.625 mg/mL inhabited alpha-glucosidase and the inhabitory rates of 16 kinds of them were 100%. Conclusion. This assay could be performed to screen α-glucosidase inhibitors by high throughput way in vitro,and some of ETCM had high inhibitory actvities for α-glucosidase,which would be important for treating diabetes mellitus.关键词
药食两用中药/α-葡萄糖苷酶/抑制剂/高通量筛选Key words
edible traditional Chinese medicine α-glucosidase inhibitor high throughput screening分类
轻工纺织引用本文复制引用
许芹永,朱靖博,宋青楠,王振中,肖伟..药食两用中药中α-葡萄糖苷酶抑制剂的高通量筛选[J].食品工业科技,2012,33(13):110-113,4.基金项目
辽宁省教育厅科学技术研究项目 （）
中药制药过程新技术国家重点实验室（SKL2010M0205,SKL2010202011）. （）
