食品工业科技2012，Vol.33Issue(13)：127-130,134,5.
醋酸纤维素／聚四氟乙烯复合膜固定化磷脂酶A1的研究
Study on phospholipase A1 immobilized by CA/PTFE composite membrane
摘要
Abstract
The phospholipase was immobilized on the cellulose acetate （CA）/polytetrafluoroethylene （PTFE） by adsorption. The single-factor tests of immobilization condition were made though investigateing lipase activity, then the results were optimized by the response surface methodology. The optimal condition of the immobilization was as foilows：phospholipase concentration 0.06g/mL,adsorption for 3h,adsorption temperature at 60℃, oscillation velocity 200r/min,the activity of immobilized lipase reached 4.0U/cm2. From the results of SEM, phospholipase were well immobilized by the CA/PTFE.关键词
醋酸纤维素／聚四氟乙烯复合膜/固定化/磷脂酶A1/响应面/SEMKey words
CA/PTFE composite membrane immobihzation/phospholipaseA response surface SEM分类
轻工纺织引用本文复制引用
时敏,周晓丹,李越,李志平,于殿宇..醋酸纤维素／聚四氟乙烯复合膜固定化磷脂酶A1的研究[J].食品工业科技,2012,33(13):127-130,134,5.基金项目
国家863高技术研究发展计划油脂非均相反应和多相分离精制技术 （）
国家十二五科技支撑计划大豆油加工关键技术和装备研究与示范（2011BAD02801）. （）
