食品工业科技2012，Vol.33Issue(13)：158-162,5.
产叶酸菌株的筛选及其发酵条件的优化
Screening of folic acid producing strains and optimization of fermentation conditions
徐玲燕 1潘秋月 2聂小华 1孟祥河1
作者信息
- 1. 浙江工业大学生物与环境工程学院,浙江杭州310014
- 2. 浙江经贸职业技术学院应用工程系,浙江杭州310018
- 折叠
摘要
Abstract
Strain R-1 and S-1 which can synthesize folic acid were isolated from soil sample. Single factor experiments and orthogonal experiments were conducted,and the optimization of fermentation conditions （carbon and nitrogen source,initial pH,fermentation time） could increase folic acid levels by approximately 10%. The addition of PABA resulted in about two-fold increase in Strain R-1 of folic acid yields,while the Strain S-1 increased 1.8-fold. The optimal fermentation conditions for strain R-1 were as follows：glucose 25g/L, ammonium citrate 4g/L,PABA 0.3g/L,inoculation volume of 2% （v/v）,initial pH 6.5,fermentation time 24h and the folic acid yield was up to 1.92μg/mL. Meanwhile,the folic acid yields by strain S-1 was 1.31μg/mL when fermentation was performed at sucrose 20g/L,ammonium citrate 2g/L,initial pH5.5 for 48h,with inoculation volume of 3%（v/v）,PABA dosa.qe of 0.3g/L.关键词
叶酸/筛选/优化/对氨基苯甲酸Key words
folic acid screening optimization/para-aminobenzoic acid (PABA)分类
轻工纺织引用本文复制引用
徐玲燕,潘秋月,聂小华,孟祥河..产叶酸菌株的筛选及其发酵条件的优化[J].食品工业科技,2012,33(13):158-162,5.
