食品工业科技2012，Vol.33Issue(13)：228-230,235,4.
条斑紫菜蛋白和多糖的逆流脉冲超声辅助提取技术研究
Study on counter-current pulsed ultrasound-assisted extraction of protein and polysaccharide from Porphyra yezoensi$
摘要
Abstract
To reduce production cycle and increase product yield ,the counter-current pulsed ultrasound technology was adopted to extract the protein and polysaccharide from Porphyra yezoensis. The Box-Behnken central composite design combined with response surface analysis was used to optimize the pulsed ultrasound-assisted extraction condition. In the study,the regression equation was got and proved through regression analysis and experimental verification. The results showed that the optimum conditions of counter- current pulsed ultrasound-assisted extraction were time of 85min,temperature of 40℃ and solid-liquid ratio of 19：1 （mg/mL） on the conditions of pH9.0,pulsed duration time of 4s and interval time of 2s. Under the optimum conditions,the total yield of protein and polysaccharide from Porphyra yezoensis could reach up to 37.26%,in which the yields of protein and polysaccharide were 15.68% and 21.58%,respectively. The total yield of protein and polysaccharide from Porphyra yezoensis increased 116.38% by using the counter-current pulsed ultrasound technology compared to the traditional extraction,which was greatly increased.关键词
条斑紫菜/逆流脉冲超声辅助提取/蛋白/多糖/响应面分析Key words
Key words/Porphyra yezoensis counter-current pulsed ultrasound-assisted extraction protein polysaccharideresponse surface analysis分类
轻工纺织引用本文复制引用
王婷,马海乐,曲文娟,吕艳歌,郑惠华..条斑紫菜蛋白和多糖的逆流脉冲超声辅助提取技术研究[J].食品工业科技,2012,33(13):228-230,235,4.基金项目
科技支撑计划一农业部分 （）
南通市产学研联合创新科技计划 （）
江苏高校优势学科建设工程资助项目. （）
