食品工业科技2012，Vol.33Issue(13)：303-305,310,4.
固相萃取-高效液相色谱-柱后衍生荧光法测定奶粉中维生素B11含量
Determination of vitamin Bll contents in powdered milk by SPE-HPLC-Postcolumn fluorescence detection
王京 1许荣年 1汪庆旗 1杨琳 1陈青俊 1管有根1
作者信息
- 1. 浙江省食品质量监督检验站,浙江杭州310009
- 折叠
摘要
Abstract
The solid phade extraction-high performance liquid chromatography-post column derivatization fluorescence detection was applied to determinate the Vitamin Bll contents in powdered milk. After the solid phade extraction purification,the moving phade of the sample on the ZORBAX SB-C18 chromatographic column was a monopotassium phosphate-acetonitrile system whose pH was 3.5,concentration was 0.05mol/L with a moving speed of 1.0mL/min and a sample size of 20μL. The derivatization reagent taken was the potassium persulfate solution with a solubility of 0.5% and the temperature of reactor was 60% with a speed of 0.5mL/min,then the sample was detected and fixed by fluorescence detector（Ex=365nm, Em=440nm,added values=100,attenuation values=16）. The result showed that the detection method worked well linearly within the range of 0.02-1.76pg/mL and R2=0.9999 with a detectability of 4ng/mL and a quantitation limitation of 12ng/mL. The sensitivity could meet the demand of powdered milk detection well. The method＇s coefficient of recovery was between 92.5%-95.7% and coefficient of variation was 1.32%.关键词
固相萃取/柱后衍生/鸡胰冻干粉/奶粉Key words
solid phade extraction/postcolumn derivatization/chicken's pancreas lyophiledpowder/powdered milk分类
轻工纺织引用本文复制引用
王京,许荣年,汪庆旗,杨琳,陈青俊,管有根..固相萃取-高效液相色谱-柱后衍生荧光法测定奶粉中维生素B11含量[J].食品工业科技,2012,33(13):303-305,310,4.
