食品工业科技2012，Vol.33Issue(13)：319-321,3.
利用线粒体DNACyt易基因PCR-RFLP分析方法鉴别羊肉和鸭肉
Identification of mutton from duck meat by PCR-RFLP analysis of mitochondrial DNA Cyt b gene
摘要
Abstract
A method to distinguish mutton of sheep and goat from duck meat was developed based on the PCR-RFLP analysis of mitochondrial DNA（mtDNA） Cyt b gene. A pair of universal PCR primers was employed to amplify a fragment of mtDNA Cyt b gene in sheep,goat and duck. The PCR products were further digested by two DNA restriction endonucleases of Bsu361 and Spel. The results showed that the primers amplified successfully a 472bp long DNA fragment from all three species. The PCR-RFLP patterns resulted from Spel digestion of both sheep and goat PCR products carried two bands in 213bp and 259bp which were different from that of duck PCR product being digested by Bsu361 into 95bp and 377bp. This study demonstrated that the PCR-RFLP analysis of mtDNA Cyt b gene was a rapid method to accurately distinguish mutton of sheep and goat from duck meat.关键词
PCR-RFLP/物种鉴别/线粒体DNA/Cyt/6基因Key words
PCR-RFLP species identification mtDNA Cyt b gene分类
轻工纺织引用本文复制引用
冯海永,刘丑生,何建文,安添午,韩建林..利用线粒体DNACyt易基因PCR-RFLP分析方法鉴别羊肉和鸭肉[J].食品工业科技,2012,33(13):319-321,3.基金项目
“十一五”国家科技支撑计划项目 （）
CAAS-ILRI联合研究项目 （）
中国农业科学院一级岗位杰出人才基金. （）
