食品与发酵工业2012，Vol.38Issue(3)：43-48,6.
枯草芽孢杆菌产中性蛋白酶的特性及补料研究
Studies on Neutral Protease Production Using Bacillus subtilis and Fed-batch Culture
摘要
Abstract
Corn flour is chosen as the best carbon source and the best nitrogen source is soybean meal,using Bacillus subtilis as the neutral protease producer.Phosphate increases the protease production.When Na_2HPO_4 and KH_2PO_4 were not added in the medium,Ca~2＋ and Mg~2＋ at the given concentration cause inhibition on protease production.Ca~2＋ and Mg~2＋ at 1 mmol/L result in 12% and 8% increase in the enzyme production,respectively,with the addition of Na_2HPO_4 and KH_2PO_4 in the medium;however,higher concentration of Ca~2＋ and Mg~2＋ have an inhibitory effect on the enzyme production.Feeding of corn flour（10g/L） and soybean meal（7.5g/L） at 11,18,24 h results in 96% increase in protease yield and 112% increase in protease productivity when compared to the values obtained for batch culture.关键词
枯草芽孢杆菌/中性蛋白酶/发酵/分批补料Key words
Bacillus subtilis/neutral protease/fermentation/fed-batch分类
化学化工引用本文复制引用
陈鸿图,傅金磊,杨月明,梁颖婕,谢文化,朱明军..枯草芽孢杆菌产中性蛋白酶的特性及补料研究[J].食品与发酵工业,2012,38(3):43-48,6.基金项目
广东省产学研项目 （）
华南理工大学2011年“百步梯攀登计划”和“学生研究计划项目”资助 （）
