食品与发酵工业2012，Vol.38Issue(3)：68-72,5.
肉桂醛特异性抑制酵母细胞壁合成的作用机理
Studies of Inhibitory Effect of Trans-cinnamaldehyde on Cell Wall Synthesis in Yeast
摘要
Abstract
The antifungal mechanism of trans-cinnamaldehyde was studied using the research pattern consisting of wild-type Saccharomyces cerevisiae strain WHU2a and three cell wall synthase-deficient strains Δfks1,CHS020 and CHS003 which were derivatives of WHU2a.The results showed trans-cinnamaldehyde had an obvious inhibition effect on glucan synthase-deficient strain Δfks1,the minimum inhibitory concentration（MIC） of trans-cinnamaldehyde was 8 μg/mL,which was 8 folds lower than that against the wild-type strain.Besides,the MIC against chitin synthase-deficient strains CHS020 and CHS003 were 4 folds lower as well.By growth rescue assay,it was proved that the growth inhibition effect of trans-cinnamaldehyde on Δfks1 could partly be rescued by sorbitol,which confirmed the susceptibility of trans-cinnamaldehyde on cell wall.Furthermore,the contents of β-glucan and chitin on Δfks1 cell wall were proved decreasing by 23.48% and 31.94%,respectively,after treated by trans-cinnamaldehyde.Based on the data presented,it is reasonable to conclude that the main antifungal mechanism of trans-cinnamaldehyde is to specifically inhibit the synthesis of β-glucan and chitin on fungal cell wall.关键词
肉桂醛/抗真菌作用/葡聚糖合酶/几丁质合酶/Saccharomyces/cerevisiaeKey words
trans-cinnamaldehyde/antifungal/β-1/3-glucan synthase/chitin synthase/Saccharomyces cerevisiae分类
农业科技引用本文复制引用
汪琨,徐峥,汪倩雯,朱廷恒,崔志峰..肉桂醛特异性抑制酵母细胞壁合成的作用机理[J].食品与发酵工业,2012,38(3):68-72,5.基金项目
浙江省自然科学基金资助项目 （）
