食品与发酵工业2012，Vol.38Issue(3)：109-114,6.
1株产耐热脂肪酶根霉突变株的选育
Selection of One Rhizopus sp.Mutant Strain High Producing Thermostable Lipase
孙春艳 1赵祥颖 2董学前 2乔君 2刘建军3
作者信息
- 1. 山东农业大学食品科学与工程学院,山东泰安271018
- 2. 山东省食品发酵工程重点实验室,山东济南250013
- 3. 山东农业大学食品科学与工程学院,山东泰安271018／山东省食品发酵工程重点实验室,山东济南250013
- 折叠
摘要
Abstract
A high enzyme activity mutant strain UD-23 was selected from a Thermostable Lipase-producing Rhizopus SFE-L01 which was stored in our laboratory by using UV,DES and UV ＋ DES mutagenetic treatments.Compare to the original strain,the mutant＇s enzyme activity increased by 1.12 times and reached 24 000.00 U/mL.Study of the production conditions and enzymatic characteristics of the lipase from UD-23 showed that the optimal temperature of the lipase from UD-23 was 60℃.After the optimization,the enzyme activity of this mutant strain reached 30 000.00U/mL,increased by 25%.关键词
耐热脂肪酶/根霉/菌种诱变Key words
thermostable lipase/Rhizopus/mutation breeding分类
化学化工引用本文复制引用
孙春艳,赵祥颖,董学前,乔君,刘建军..1株产耐热脂肪酶根霉突变株的选育[J].食品与发酵工业,2012,38(3):109-114,6.
