反相高效液相色谱法测定苹果酸发酵液中的有机酸OA北大核心CSCDCSTPCD
Determination of Organic Acids in Malic Acid Fermentation Liquor by Reversed Phase HPLC Method
采用反相高效液相色谱法分析测定苹果酸发酵液中的有机酸，最终确定了适用于发酵液中各种有机酸含量测定的色谱条件，色谱柱：COSMOSIL 5C18-PAQ（4．6mmI．D．×250mm）；紫外检测波长：210nm；流动相：1％乙腈-20mmol／L KH2PO4溶液（pH2．8）；流速：O．5mL／min；柱温：23℃。结果表明，在该条件下苹果酸发酵液中的7种有机酸都得到了较好分离，方法的回收率为94．2％-102．7％，表明具有较好的准确度和精密度。
Reversed phase HPLC method was applied to determine organic acid in malic acid fermentation liquor in this paper. The chromatographic conditions are finally conformed to apply to all kinds of organic acid content deter-mination in the fermentation. The conditions are as follows： COSMOSIL 5C18 - PAQ column （4.6 mm I.D. × 250 mm） was used at 23℃. UV detection wavelength was 210 nm. Solution containing 1% acetonitrile and 20mmol/L KH2PO4 at pH 2.8 was used as m…查看全部>>
黄慧敏;张伟国
江南大学工业生物技术教育部重点实验室,江苏无锡214122江南大学工业生物技术教育部重点实验室,江苏无锡214122
轻工纺织
反相高效液相色谱法苹果酸发酵液有机酸
reversed phase HPLCmalic acid fermentation liquororganic acids
《食品与发酵工业》 2012 (5)
180-184,5
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