食品与发酵工业2012,Vol.38Issue(5):223-227,231,6.
橘皮中橙皮苷提取的优化及其对羟自由基清除作用
Optimization of Total Hesperidin Extraction from Citrus Peel by Response Surface Methodology and Its Scavenging Activity on Free Hydroxyl Radial
摘要
Abstract
Four factors including ethanol concentration, solvent to material ratio, extraction temperature and ex-traction time were studies on the yield of hesperidin from the citrus peel by using single factor analysis method. Then a four factor, three-level Box-Behnken central composite design (CCD) coupled with response surface analysis (RSM) was applied in optimization of the extraction. It was found that the optimum extraction conditions were : etha-nol concentration 71% , solvent to material ratio 22:1 (mL/g) , extraction temperature 70℃ and extraction time 2.5 h. Under the optimized extraction conditions, hesperidin extraction yield was 4.82%. The research on anti-oxidation properties showed that the extracted hesperidin had scavenging activity on free hydroxyl radical.关键词
响应曲面/橙皮苷/提取工艺/羟自由基Key words
response surface methodology/hesperidin/extraction technological conditions/hydroxyl free radical分类
轻工纺织引用本文复制引用
黄芳,周宏,陈华,扶庆权..橘皮中橙皮苷提取的优化及其对羟自由基清除作用[J].食品与发酵工业,2012,38(5):223-227,231,6.基金项目
国家自然科学基金 ()
重庆市自然科学基金(2010BB0063)资助 ()