食品与机械2012，Vol.28Issue(2)：52-55,65,5.
芝麻过敏原PCR检测方法
Establishment of PCR method for detecting sesame allergen
摘要
Abstract
Sesame is an important food allergen;low-level doses can cause severe allergic reactions. In this paper, PCR primers were designed based on sesame allergy protein (2S albumin) gene sequence, and conventional PCR and Real-time PCR were established and optimized. The lowest DNA content that can be detected for conventional PCR was 0. 1 ng and 0, 01 ng for real-time PCR, respectively. The specificity of the methods is good and can be widely used.关键词
芝麻/过敏原/聚合酶链式反应/实时荧光定量聚合酶链式反应/2S白蛋白Key words
sesame/ allergen/PCR/Real-time PCR/2S albumin引用本文复制引用
张文举,许庆金,邓志瑞,陈沁..芝麻过敏原PCR检测方法[J].食品与机械,2012,28(2):52-55,65,5.
