食品与机械2012,Vol.28Issue(2):147-151,5.
鲢鱼蛋白制备ACE抑制肽的工艺优化
Optimization on preparation of ACE-Inhibitory peptide from hypohythalmichthys molitrix protein
摘要
Abstract
The study was to seek the optimum conditions of producing ACE inhibitory peptide from Hypohythalmichthys molitrix protein. Determination of ACE-inhibition rate of various hydrolysates using high performance liquid chromatography, the best flavor proteinase was screened out. Based on single-factor experiments, the response surface methodology model was arranged according to Box~Beknken central composite experiment design. The results showed that the optimum preparation ACE-inhibitory peptide from Hypohythalmichthys moiitrix protein conditions were: original pH 7. 54, temperature 50. 56℃, time 4. 5 h, water addition 46. 67 mL Under the above condition, the ACE-inhibition rate can reach up to 81. 35%.关键词
鲢鱼/ACE抑制肽/响应面/高效液相色谱Key words
hypohythalmichthys molitrix/ACE-inhibitory peptide/response surface methodology/high performance liquid chromatography引用本文复制引用
尹歆,邓放明..鲢鱼蛋白制备ACE抑制肽的工艺优化[J].食品与机械,2012,28(2):147-151,5.基金项目
湖南省重大科技专项(编号:2010FJ1007-2) (编号:2010FJ1007-2)