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鲢鱼蛋白制备ACE抑制肽的工艺优化

尹歆 邓放明

食品与机械2012,Vol.28Issue(2):147-151,5.
食品与机械2012,Vol.28Issue(2):147-151,5.

鲢鱼蛋白制备ACE抑制肽的工艺优化

Optimization on preparation of ACE-Inhibitory peptide from hypohythalmichthys molitrix protein

尹歆 1邓放明2

作者信息

  • 1. 食品科学与生物技术湖南省重点试验室,湖南长沙410128
  • 2. 湖南农业大学食品科学技术学院,湖南长沙410128
  • 折叠

摘要

Abstract

The study was to seek the optimum conditions of producing ACE inhibitory peptide from Hypohythalmichthys molitrix protein. Determination of ACE-inhibition rate of various hydrolysates using high performance liquid chromatography, the best flavor proteinase was screened out. Based on single-factor experiments, the response surface methodology model was arranged according to Box~Beknken central composite experiment design. The results showed that the optimum preparation ACE-inhibitory peptide from Hypohythalmichthys moiitrix protein conditions were: original pH 7. 54, temperature 50. 56℃, time 4. 5 h, water addition 46. 67 mL Under the above condition, the ACE-inhibition rate can reach up to 81. 35%.

关键词

鲢鱼/ACE抑制肽/响应面/高效液相色谱

Key words

hypohythalmichthys molitrix/ACE-inhibitory peptide/response surface methodology/high performance liquid chromatography

引用本文复制引用

尹歆,邓放明..鲢鱼蛋白制备ACE抑制肽的工艺优化[J].食品与机械,2012,28(2):147-151,5.

基金项目

湖南省重大科技专项(编号:2010FJ1007-2) (编号:2010FJ1007-2)

食品与机械

OA北大核心CSCDCSTPCD

1003-5788

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