食品与机械2012，Vol.28Issue(3)：80-83,4.DOI:10.3969/j.issn.1003-5788.2012.03.022
重金属铅酶联免疫检测方法的研究
Development of enzyme-linked immunosorbent assay for heavy metal lead
杨依锦 1王俊平 2王津 1方淑兵 2王硕1
作者信息
- 1. 食品营养与安全教育部重点实验室,天津300457
- 2. 天津科技大学,天津300457
- 折叠
摘要
Abstract
To prepare polyclonal antibody against heavy metal lead ion and develop an indirect competitive ELISA method. Lead was coupled to vector proteins keyhole limpet hemocyanin( KLH) and bovine serum albumin(BSA) via a bifunctional chelating agent 1TCBE respectively, Pb(D HTCBE-KLH was used as immunogen and Pb( II)-ITCBE-BSA was used as coating antigen. The anti-Pb antibody was obtained after immunized to New Zealand white rabbits and an indirect competitive ELISA method was developed. by which the determination result was compared with that by ICP-MS method. The standard curve was developed with JC50 of 3. 48 ng/mL and IC15 of 0. 32 ng/mL, the total coincidence rate of the developed ELISA method and ICP-MS method was more than 97%.关键词
铅/多克隆抗体/间接竞争ELISAKey words
heavy metal lead/polyclonal antibody/ic-ELISA引用本文复制引用
杨依锦,王俊平,王津,方淑兵,王硕..重金属铅酶联免疫检测方法的研究[J].食品与机械,2012,28(3):80-83,4.
