食品与机械2012，Vol.28Issue(3)：84-86,3.DOI:10.3969/j.issn.1003-5788.2012.03.023
间接竞争酶联免疫法检测水样中的重金属镉
Development of heavy metal cadmium in water samples by indirect competitive enzyme-linked immunosorbent assay
王津 1生威 1王俊平 1方淑兵 1杨依锦 1张曙光 1王硕1
作者信息
- 1. 天津科技大学食品营养与安全教育部重点实验室,天津300457
- 折叠
摘要
Abstract
An indirect competitive enzyme-linked immunosorbent assay was developed to low instrument cost detect cadmium in water samples using the polyclonal antibody. The limit of detection (JC15) was 0. 76μg/L, and the sensitivity (IC50) was 11.33 μg/L. The cross-reactivity rates of the antibody with other metals(Mn, Cr, Mg, Fe, Pb, Ni, Ag and Cu) chelates were below 1.32%. except for Hg-chelate with a cross-reactivity rate 10. 9%. The recoveries of cadmium in spiked tap water and river water were 93. 95%~107. 40%, and the coefficients of variation were 3. 97%~14. 69%.关键词
镉/重金属免疫学检测法/多克隆抗体/酶联免疫法/自来水/河水Key words
cadmium/ heavy metals immunological detection method/polyclonal antibodysELISA/tap water/river water引用本文复制引用
王津,生威,王俊平,方淑兵,杨依锦,张曙光,王硕..间接竞争酶联免疫法检测水样中的重金属镉[J].食品与机械,2012,28(3):84-86,3.
